CasBLASTR is a tool maintained by the Joung Lab
to make designing CRISPR-Cas9 constructs for targeted genome editing as easy as possible.
How does it work?
CasBLASTR takes an input sequence that you may want to target, the type of Cas9 protein you wish to use, your 5' guide RNA design restrictions, and your desired spacer lengths to find all possible target sites.
Once CasBLASTR produces a list of possible targetsites, you can sort and select from the table of outputs. You are then given the option of exporting either all or just the selected results to a